本研究表明了 FATIMA 在快速分析细胞侵入两维和三维基质能力上有着卓越的适用性，在研究固定和悬浮细胞时都可以适用。 FATIMA 提供了可更精确和更具重复性地研究可通过简单或复杂 ECM 基质（或类似的三维基质）的细胞数量的方法 。侵入速度可以由单个的终点或不同侵入过程的动力学时间点参数进行测量。

(1) TERRANOVA, V.P., HUJANEN, E.S., LOEB, D.M., MARTIN, G.R., THORNBURG, L., & GLUSHKO, V. : Use of a reconstituted basement membrane to measure cell invasivness and select for highly invasive tumor cells. Proc. Natl. Acad. Sci. USA 83 , 465-469 (1989).

(2) SCHONEMARK, M.P., BOCK, O., BUCHNER, A., STEINMEIER, R., BENBOW, U., & LENARZ, T.: Quantification of tumor cell invasion using confocal laser scan microscopy. Nature Med . 3 , 1167-1171.

(3) ALBINI, A., IWAMOTO; Y., KLEINMAN, H.K., MARTIN, G.R., AARONSON, S.A., KOZLOWSKI, J.M., & McEWAN, R.N.: A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res . 47 , 3239-3245 (1987).

(4) WELCH, D.R., LOBL, T.J., SEFTOR, E.A., WACK, P.J., AEED, P.A., YOHEM, K.H., SEFTOR, R.E.B., & HENDRIX, M.J.C.: Use of the membrane invasion culture system (MICS) as a screen for anti-invasive agents. Int. J. Cancer 43 , 449-457 (1989).

(5) REPESH, L.A.: A new in vitro assay for quantitating tumor cell invasion. Invasion

Metast . 9 , 192-208 (1989).

(6) GEHLSEN, K.R. & HENDRIX, M.J.C.: In vitro assay demonstrates similar invasion profiles for B16F1 and B16F10 murine melanoma cells. Canc. Lett . 30 , 207-212 (1993).

(7) MUIR, D., SUKHU, L., JONHSON, J., LAHORRA, A., & MARIA, B.L.: Quantitative methods for scoring cell migration and invasion in filter-based assays. Anal. Biochem . 215 , 104-109 (1993).

附： FATIMA测定细胞侵入的基本步骤示意图