RNA电泳

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?         RNA Gel (Crawford Lab) ?         Gel Electrophoresis of RNA (Beverly Faulkner-Jones) great tips on RNA gel electrophoresis.  ?         Northern Gel and  Transfer (gopher://iubio.bio.indiana.edu) Using glyoxalated RNA ?         RNA Electrophoresis (Molecular Genetics RecLab) ?         RNA Electrophoresis in Denaturing Formaldehyde Gel (Molecular Genetics Rec Lab) ?         Formaldehyde-Agarose Gels (Beverly Faulkner-Jones) Agarose gels have a smaller resolving power than polyacrylamide gels but a greater range of separation - from 200 bp to >50 kb using standard gels and electrophoresis equipment. RNAs up to 10 000 kb can be separated in agarose gels using pulsed field gel electrophoresis. ?         Denaturing polyacrylamide gel electrophoresis of RNA (Beverly Faulkner-Jones) Polyacrylamide gels have enough resolving power to separate fragments differing by only one base pair in size, but their range is ~ 5 to 1000 bp. They are much more difficult to handle than agarose gels. ?         Recovery of RNA fragments from polyacrylamide gels (Beverly Faulkner-Jones) The best method is the crush and soak method. It can be sued to isolate single-stranded RNA from denaturing gels. The RNA is of high purity but the method is long and inefficient if long transcripts are to be recovered (cf. less than 30% of DNA greater than 3 kb is recovered). Polyacrylamide Gel Electrophoresis (PAGE) of RNA (Frank Bottone) For use withRibonuclease Protection Assay (RPA): 处理 SSI 文件时出错