DNA的诱变和甲基化

作者：佚名    实验频道来源：中华基因网    点击数：    更新时间：2004-6-8

?         In Vitro Mutagenesis Using Altered Sites (Bowtell Lab)  In vitro Mutagenesis with dut ung single stranded DNA (Hahn Lab) ?         Site-directed Mutagenesis using PCR (Lazo Lab) PCR site-directed methods allow site-specific mutations to be incorporated in virtually any double-stranded plasmid; eliminating the need for M13-based vectors or single-stranded rescue. ?         Site-directed mutagenesis (Dr. Chastain) ?          Site Directed Mutagenesis Using the Ung-Dut System (Bowtell Lab) ?         Oligonucleotide-directed mutagenesis (Kunkel method) (Jeff NEwman) ?         Site-directed Mutagenesis (Promega) For generation and selection of oligonucleotide-directed mutants ?         Site Directed Mutagenesis Using the Ung-Dut System (PMCI Research) ?         Nested Deletions (Bowtell Lab) Nested Deletions using exonuclease-III and mung bean nuclease ?         Deletion Mutagenesis (Promega) For construction of plasmid or M13 subclones containing progressive unidirectional deletions of  inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation,  transformation... ?         ExoIII/S1 Deletion (Fermentas) For construction of plasmid or M13 subclones with nested unidirectional deletions. The method is applicable for DNA sequencing, mapping of boundaries of regions involved in genetic control, DNA/protein interaction sites, or protein domains. ?         Exo/S1 Deletion Series (Vesicle Trafficking)   Four Primer Mutagenesis (PDF) (Templenton Lab) A PCR-based mutagenesis, based on Sarkar and Sommer, but with no ligation required.  ?         Nested Deletions Using Exonuclease-III and Mung Bean Nuclease (PMCI Research) ?         In vitro Mutagenesis Using Altered Sites (PMCI Research) ?         Unidirectional deletions (erase-a-base) (Crawford Lab)   Design of Mutagenic Oligonucleotides (Promega) Methylation analysis ?         Bisulfite Treatment of DNA (Issa Lab) ?         Methylation-Sensitive PCR (MSP) (Issa Lab) ?         Bisulfite-PCR (for restriction and/or sequencing) (Issa Lab) ?         Southern Blot Analysis (Issa Lab) ?         Methylated CpG Island Amplification (MCA) (Issa Lab) ?         DNA-Methyltransferase Assay (Issa Lab) ?         Bisulfite Modification (TTO) Modify DNA with bisulfite for methylation analysis 处理 SSI 文件时出错