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- Biosynthetic labeling (Sefton Lab)
- Biotinylation of Antibody (Contributed by Nanci Donacki)
- 125I Labeling of Protein using ICl (ScienceXchange)
- Protein (Antibody) Fluorescent Labeling (Mario Roederer)
Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
- Fluorescein (FITC)
- Phycoerythrin (PE)
- Cy5PE
- Cy7PE
- Texas Red (TR)
- Allophycocyanin (APC)
- Cy5
- Cy7APC
- Cascade Blue
- Biotin
In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of non-immunoglobulin proteins.
- FITC
- PE
- TR-BSA
- APC
- Cascade Blue
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