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细菌检测-实验方法 |
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来源：互联网 作者：未知 发布时间：2006-12-16
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- Gram Staining (+\-) (William H. Heidcamp)
- Gram-Staining Procedure (MEDIC, U of Texas)
Very nice and detailed method description for Gram staining
- Acid-Fast Stain (Putt's Method)
To detect the presence of acid-fast mycobacteria in tissue sections
- Smear Preparation (MEDIC, U of Texas)
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.
- Alkaline Phosphatase and Phospholipase C Assay (Hancock Lab) (Accessible only by Internet Explorer)
- Bacteria Agglutination Assay (Hancock Lab) (Accessible only by Internet Explorer)
Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120oC for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cells grown to mid log phase, then centrifuged and resuspended in saline.
- Carbohydrate Assay(Hancock Lab) (Accessible only by Internet Explorer)
This assay is used to determine the relative amounts of LPS CHO present in a given strain and can be done on one set of samples and then scanned at the usr/localious wavelengths for reasonable data on the 3 types of sugars.
- Dintrophenylation Assay for Polymixin B (Hancock Lab) (Accessible only by Internet Explorer)
- Endotoxin Assay(Hancock Lab) (Accessible only by Internet Explorer)
This assay measures the shift in the absorbance maximum of the dye due to binding to endotoxin
- Indirect Immunfluoresence (Hancock Lab) (Accessible only by Internet Explorer)
- Liposome Swelling Assay (Hancock Lab) (Accessible only by Internet Explorer)
Also including method for liposome Isolation and liposome preparation
- Preparation of E Coli Protein for SDS-PAGE (Hahn Lab)
Simplified method for analyzing E coli protein
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