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细菌人工染色体-实验方法 |
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来源：互联网 作者：未知 发布时间：2006-12-16
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- The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript) (Clemson University Genomics Institute)
- Construction of BAC Library (Caltech)
Guideline for BAC library preparation, the followings are described in great detail
- Preparation of BAC vector DNA
- Preparation of source DNA
- Ligation and Electroporation
- Preparation of BAC DNA
- BAC Manual (Texas A&M BAC Center)
Provide details in the theory and practice for the construction, characterization, and screening of BAC libraries.
- Introduction
- MegaBase DNA Preparation
- BAC Vector Preparation
- Solutions
- Hybridization of High Density Arrayed BAC Nylon Filter Blots (Caltech)
Procedures for isolation and purification of cDNA inserts using polymerase chain reaction, probe labeling, probe hybridization and more...
- BAC/PAC/Fosmid Prep(WUGSC)
- BAC Sequencing with ET primers(WUGSC)
- BAC End-Sequencing (Caltech)
Prepare BAC DNA for sequencing
- Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation (Roe Lab)
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