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酵母转化-实验方法
来源:互联网 作者:未知 发布时间:2006-12-16
·         Yeast Transformation (Gietz Lab)
LiAc/SS-DNA/PEG Transformation
·         Yeast Transformation (Breeden Lab)
LiAc method
·         Large-Scale Yeast Transformation (Yale Yeast Analysis Center)
Detailed protocol for large-scale yeast transformation including competent cell preparation, transfromation...
·         Yeast Transformation (Herskowitz Lab)
·         Sleazy Plate Transformation of Yeast (Herskowitz Lab)
·        Transformation of Yeast - LiAc Method (Lazo Lab)  
·        Two Hybrid System Transformation (Gietz Lab)
This  Protocol is for 2 Hybrid system screens
·         Yeast Transformation (Fangman/Brewer lab)
Yeast transformation protocol  Using Frozen Competent Cells and Single-stranded DNA as a Carrier
·         Quick Yeast Transformation (Gietz Lab)
This Protocol allows for transformation with any Yeast cell source. When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. This procedure is very flexible and can be applied to yeast cells from a number of different sources; however, for best results, use cells from a freshly grown plate.
·        Yeast Transformation (D. Amberg)
Electroporation method
·        Fission Yeast Transformation (Forsburg Lab) 
Includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion. 
·        Multiwell Transformation  (Davis Lab)
Detailed protocol with LiAcetate PEG transformation method
·        Transformation of Yeast (PMCI Research)
·        "Lazy Bones" Plasmid Transformation (Dohlman Lab) 
A quick method for plasmid transformation into yeast.
·        Competent Yeast Preparation (Gietz Lab)
This Protocol allows you to prepare frozen yeast cells that are competent for transformation after thawing
·         Yeast Transformation (Gottschling Lab)
Provides LiAc method, "lazy bones" method and electroporation method 
·        Yeast Transformation (Finley Lab) 
LiOAc method
  • Yeast Transformation with DMSO/LioAc (high efficiency) (Hahn Lab)
      
  • Yeast Transformation (Rapid method) (Hahn Lab)
    LiOAc method
·         Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue (Gottschling Lab)
·        Yeast Colony PCR (Gottschling Lab)
For yeast transformant screening
·        Direct PCR from Whole Yeast Cells (Namjin Chung)
Zymolyase Method for screening yeast transformants

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