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DNA指纹法-实验方法
来源:互联网 作者:未知 发布时间:2006-12-16
  • DNA Fingerprinting (David F. Betsch)
    the theory, procedures and applications
      
?         DNA Fingerprinting (Polyarylamide Gel) (Caltech)
BAC DNA sample and gel preparation, gel running and more
  
?         DNA Fingerprinting (Agarose Gel) (Caltech)
  
?         AFLP (Mannie Liscum and Paul Oeller)
AFLP was designed as a highly sensitive method for DNA fingerprinting to be used in a usr/localiety of fields, including plant and animal breeding, medical diagnostics, forensic analysis and microbial typing, to name a few. 
DNA Amplification Fingerprinting (DAF) 
  • DNA Amplification Fingerprinting (DAF)  by (DNA KAFFE)
    DAF is a general nucleic acid amplification technique that uses at least one primer of at least 5 nucleotides (nt) in length to produce characteristic and highly informative DNA patterns.  This protocol describes the DAF technique in detail, highlighting the role of primer design and appropriate optimization of experimental usr/localiables.
      
  • DNA Amplification Fingerprinting Protocol (C. S. Prakash)
    DNA is amplified by PCR. The resulting PCR products are separated on polyacrylamide gel and silver stained for DNA visualization.   
      
  • Fingerprint Tailoring by (DNA KAFFE)
    The concept of "fingerprint tailoring" was introduced some time ago to depict those strategies which modify fingerprint pattern. This protocol describes some of these fingerprinting alternatives. 
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