Preparation of LB Plate (Dr. Chastain) prepare LB plate with or without antibiotics Bacterial Culture Media Recipes (WUGSC) M9 Plate Supplement (Gottschling Lab) Bacterial Media Solutions and Stocks (Donis keller Lab) LB Media Preparation for E.coli
Northern杂交 ? Southerns, Westerns, and Northerns: Molecular Cloning Techniques (Brian White, MIT) Basic theory, definition and procedures with illustration RNA Gel Preparation ? RNA Gel (Crawford Lab) How to prepare RNA gel ? Glyoxal/DMSO Gel (Carl
蛋白质提取和纯化（主要内容如下） Protein Extraction Protein Purification Protein Precipitation Column Preparatioin Q A Posted in the Method Forum Protein Extraction Whole Cell Lysate Preparation (UCSF Tumor Immunology) For preparing proteins to be s
蛋白质电泳（主要内容如下） One-Dimensional SDS-PAGE Two-Demensional SDS-PAGE Protein Electrophoresis in Agarose Gel Gel Staining Recipes One-Dimensional SDS-PAGE ? Protein Gel and Staining (Gottschling Lab) Provides procedures for gel preparation, g
Western 杂交（主要内容如下） Preparing of Protein Lysates Western Blotting Far Western Blotting Semi Dry Blotting Stripping Membranes Trouble Shooting and Others Preparing Protein Lysates Preparing Protein Lysates for Western Blotting (Antisense Res
Yeast Two-Hybrid System (Finley Lab) This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with introduction to its background. The following procedures are described: mating assay - small scale for tens of di
Tyrosine Kinase Assay Using Synthetic Peptides (T. Miller) Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized cells or for enzyme kinetic studies. This is a detailed pro
? Analysis of Protein by Immunoprecipitatioin (P.J. Hansen Lab) This protocol describes two methods (protein A and G) in great details as well as the principal, application and trouble shooting of immunoprecipitation. ? ECM Immunoprecipitation (LTI)
Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups New This is the standard Ellmans Test for the determination of free thiols. It works well for small peptides and proteins synthesized using standard solid phase synthetic methods. (Co
