High Resolution Genetic Footprinting (Stanford) Genetic footprinting is a technique for high-resolution mapping of the functional organization of a cloned gene. An in vitro transposition reaction with purified retroviral integrase is used to generat
HeLa Cell Nuclei Preparation (John Garland) Prepare nuclear extract from HeLa cell ? Extract Preparation (Brent Graveley) Preparation of nuclear extract and cytoplasmic extract ? Pol II yeast nuclear extract prep (small scale) (Hahn Lab)
Interaction Trap/Trap Two-Hybrid System ? Yeast Two-Hybrid System (Finley Lab) This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with introduction to its background. The following procedures are described:
