The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript) (Clemson University Genomics Institute) Construction of BAC Library (Caltech) Guideline for BAC library preparation, the followings are described in great deta
DNA Fingerprinting (David F. Betsch) the theory, procedures and applications ? DNA Fingerprinting (Polyarylamide Gel) (Caltech) BAC DNA sample and gel preparation, gel running and more ? DNA Fingerprinting (Agarose Gel) (Caltech) ? AFLP (Mannie Lisc
Easy YAC Preparation Method (Andrew Davies,Shaw lab) Screening YAC libraries (Donis Keller Lab) This is a method for screening YAC libraries for specific DNA sequences. Transformants are patched onto selective medium plates and colonies lifted to ny
Gram Staining (+-) (William H. Heidcamp) Gram-Staining Procedure (MEDIC, U of Texas) Very nice and detailed method description for Gram staining Acid-Fast Stain (Putt's Method) To detect the presence of acid-fast mycobacteria in tissue sections Sme
Biosynthetic labeling (Sefton Lab) Biotinylation of Antibody (Contributed by Nanci Donacki) 125 I Labeling of Protein using ICl (ScienceXchange) Protein (Antibody) Fluorescent Labeling (Mario Roederer) Gives detailed protocol for conjugation of prot