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    <title>双向电泳完整操作步骤</title>
    <link>http://www.bioop.com/html/biocz/2007022217744.html</link>
    <description>（一）第一向等电聚焦 1. 从冰箱中取-20℃冷冻保存的水化上样缓冲液（I）（不含DTT，不含Bio-Lyte）一小管（1ml/管），置室温溶解。 2. 在小管中加入0.01g DTT， Bio-Lyte 4-6、5-7各2.5ml，充分混匀。 3. 从小管中取出400ml水化上样缓冲液，加入100ml样品，充分混匀。</description>
    <pubDate>2007-02-22</pubDate>
    <category>实验操作</category>
    <author>秩名</author>
    <comments>生物操作专门站</comments>
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<item>
    <title>琼脂糖电泳步骤之超级基础篇</title>
    <link>http://www.bioop.com/html/biocz/2007022217743.html</link>
    <description>琼脂糖电泳步骤之超级基础篇 一、电泳前准备 准备内容 作用 1. 刷干净电泳制胶的梳子，板子，槽子，蒸馏水洗净晾干 防止不必要的重复污染，减少外来的污染。梳子干净有利于梳孔的形成。 2. 检查电泳槽，根据情况更换 buffer 排除电泳槽的电极接触不良，确保 buffer 的</description>
    <pubDate>2007-02-22</pubDate>
    <category>实验操作</category>
    <author>秩名</author>
    <comments>生物操作专门站</comments>
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<item>
    <title>How easy it is to perform a DNA separation</title>
    <link>http://www.bioop.com/html/biocz/2007022217742.html</link>
    <description>A. Preparation of DNA gel: Position the gel tray on an even surface and place the blue gel casting gates in the slots provided. Combine agarose powder and running buffer (1 x TAE or 1 x TBE) in an Erlenmayer flask. Melt agarose mixture in a microwav</description>
    <pubDate>2007-02-22</pubDate>
    <category>实验操作</category>
    <author>秩名</author>
    <comments>生物操作专门站</comments>
</item>
<item>
    <title>Agilent1100液相基本操作步骤</title>
    <link>http://www.bioop.com/html/biocz/2006121616078.html</link>
    <description></description>
    <pubDate>2006-12-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>流式细胞仪Annexin V凋亡试剂盒一般染色检测方法</title>
    <link>http://www.bioop.com/html/biocz/2006101913442.html</link>
    <description></description>
    <pubDate>2006-10-19</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>PCR专题</title>
    <link>http://www.bioop.com/html/biocz/2006101813371.html</link>
    <description>介绍PCR相关的知识和应用,包括:PCR理论与方法、PCR技术应用以及其他的相关知识</description>
    <pubDate>2006-10-18</pubDate>
    <category>实验操作</category>
    <author>admin</author>
    <comments>本站</comments>
</item>
<item>
    <title>大肠杆菌电转化感受态细胞制备经验过程</title>
    <link>http://www.bioop.com/html/biocz/2006101813370.html</link>
    <description></description>
    <pubDate>2006-10-18</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>人类一些遗传性状的调查和分析</title>
    <link>http://www.bioop.com/html/biocz/2006101213225.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>小鼠SRY等基因的PCR扩增</title>
    <link>http://www.bioop.com/html/biocz/2006101213224.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>动物基因组DNA提取、电泳检测、纯度测定</title>
    <link>http://www.bioop.com/html/biocz/2006101213223.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>利用基因组原位杂交比较不同物种亲缘关系</title>
    <link>http://www.bioop.com/html/biocz/2006101213222.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>植物染色体组型和带型分析</title>
    <link>http://www.bioop.com/html/biocz/2006101213221.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>两栖动物血细胞培养的染色体观察</title>
    <link>http://www.bioop.com/html/biocz/2006101213220.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>动物染色体组型分析</title>
    <link>http://www.bioop.com/html/biocz/2006101213219.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>环境致突变因子对生物遗传物质的损伤</title>
    <link>http://www.bioop.com/html/biocz/2006101213218.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>小鼠骨髓细胞染色体标本制作[细胞遗传]</title>
    <link>http://www.bioop.com/html/biocz/2006101213217.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>两栖类骨髓细胞染色体制作方法实验</title>
    <link>http://www.bioop.com/html/biocz/2006101213216.html</link>
    <description></description>
    <pubDate>2006-10-12</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>ELISA</title>
    <link>http://www.bioop.com/html/biocz/200609171906.html</link>
    <description></description>
    <pubDate>2006-09-17</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>流式细胞术精细</title>
    <link>http://www.bioop.com/html/biocz/200609171904.html</link>
    <description></description>
    <pubDate>2006-09-17</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>流式细胞仪实验方法</title>
    <link>http://www.bioop.com/html/biocz/200609171903.html</link>
    <description></description>
    <pubDate>2006-09-17</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>单克隆抗体的制备</title>
    <link>http://www.bioop.com/html/biocz/200609161895.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>免疫细胞凋亡检测</title>
    <link>http://www.bioop.com/html/biocz/200609161894.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>免疫印迹（Western blotting）</title>
    <link>http://www.bioop.com/html/biocz/200609161893.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>细胞因子检测技术</title>
    <link>http://www.bioop.com/html/biocz/200609161892.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>免疫组织化学实验</title>
    <link>http://www.bioop.com/html/biocz/200609161891.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>免疫血清的制备及免疫球蛋白的提取</title>
    <link>http://www.bioop.com/html/biocz/200609161890.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>干扰素测定实验</title>
    <link>http://www.bioop.com/html/biocz/200609161889.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>酶联免疫吸附实验（ELISA）</title>
    <link>http://www.bioop.com/html/biocz/200609161888.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>新型报告载体pRSET-EGFP的构建及鉴定</title>
    <link>http://www.bioop.com/html/biocz/200609161887.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>聚合酶链式反应（PCR）扩增DNA片段</title>
    <link>http://www.bioop.com/html/biocz/200609161886.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>重组质粒的转化、筛选和鉴定</title>
    <link>http://www.bioop.com/html/biocz/200609161885.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>大肠杆菌杂交</title>
    <link>http://www.bioop.com/html/biocz/200609161884.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>目的基因的亚克隆</title>
    <link>http://www.bioop.com/html/biocz/200609161882.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>目的基因的获得</title>
    <link>http://www.bioop.com/html/biocz/200609161881.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
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    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>多糖的分离纯化、分子修饰及生物活性的研究</title>
    <link>http://www.bioop.com/html/biocz/200609161880.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
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    <comments>互联网</comments>
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<item>
    <title>维生素B2和维生素C的含量测定</title>
    <link>http://www.bioop.com/html/biocz/200609161879.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
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    <comments>互联网</comments>
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<item>
    <title>脂肪转化成糖的定性实验</title>
    <link>http://www.bioop.com/html/biocz/200609161878.html</link>
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    <pubDate>2006-09-16</pubDate>
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    <comments>互联网</comments>
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    <title>脂肪酸的β-氧化</title>
    <link>http://www.bioop.com/html/biocz/200609161877.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
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    <comments>互联网</comments>
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    <title>氨基转移反应的定性鉴定</title>
    <link>http://www.bioop.com/html/biocz/200609161876.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
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    <author>未知</author>
    <comments>互联网</comments>
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    <title>肌糖原的酵解作用</title>
    <link>http://www.bioop.com/html/biocz/200609161875.html</link>
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    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
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    <comments>互联网</comments>
</item>
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    <title>酵母RNA的提取及含量测定（地衣酚显色法）</title>
    <link>http://www.bioop.com/html/biocz/200609161874.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
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    <comments>互联网</comments>
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    <title>紫外吸收法测定核酸含量</title>
    <link>http://www.bioop.com/html/biocz/200609161873.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
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</item>
<item>
    <title>动物总RNA的提取及含量测定</title>
    <link>http://www.bioop.com/html/biocz/200609161872.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
    <author>未知</author>
    <comments>互联网</comments>
</item>
<item>
    <title>动物基因组DNA、总RNA的提取及含量测定</title>
    <link>http://www.bioop.com/html/biocz/200609161871.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
    <category>实验操作</category>
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    <comments>互联网</comments>
</item>
<item>
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    <link>http://www.bioop.com/html/biocz/200609161870.html</link>
    <description></description>
    <pubDate>2006-09-16</pubDate>
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    <comments>互联网</comments>
</item>
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    <description></description>
    <pubDate>2006-09-16</pubDate>
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    <description></description>
    <pubDate>2006-09-15</pubDate>
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    <pubDate>2006-09-15</pubDate>
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    <pubDate>2006-09-15</pubDate>
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